目的 比较银翘类方有效组分含量在不同制备工艺过程链中变化规律,探究影响银翘类方有效组分工艺过程的关键因素。方法 采用HPLC,测定银翘解毒片、银翘解毒颗粒、银翘解毒软胶囊不同工艺得到提取物中有效组分的含量,比较3种制剂工艺中有效组分过程链中变化情况,并采用层数分析法确立权重,进行对不同工艺进行多指标优选的综合评价。结果 银翘方在浓缩-干燥环节有效成分的损失率最大。银翘解毒片与银翘解毒软胶囊制剂制备过程中的中间体——提取液、浓缩液、干膏中的主要成分含量相差不大,但与银翘解毒颗粒制剂相差较大。在整个提取、浓缩和干燥过程链中绿原酸、甘草苷、甘草酸、连翘酯苷A损失率较其他成分更大。用层数分析权重法综合评价3种工艺,结果综合分最高的是银翘解毒软胶囊、其次银翘解毒片,最小的为银翘解毒颗粒。结论 银翘方提取-浓缩-干燥过程链中的关键环节是浓缩-干燥环节。3种制剂工艺,银翘解毒片制法中绿原酸的保留率最高,综合评价提示银翘解毒软胶囊的制备工艺相对银翘解毒片、银翘解毒颗粒工艺更有利于其他有效成分的保留。
Abstract
OBJECTIVE To investigate the key factors in the preparation process of yinqiao analogus preparations by comparing the contents of active ingredients after different preparation processes. METHODS HPLC was employed to determine the contents of the active ingredients, and the changes of the active ingredients in the extracting-concentrating-drying process chain were investigated. Analytic hierarchy process (AHP) was used to determine the weighted coefficient for comprehensive evaluation of the multiple ingredients for different preparations. RESULTS The highest loss rate of active ingredients occurred in the concentrating-drying process. The contents of the active ingredients in soft capsules and tablets were similar in the extraction, concentration and drying processes and all higher than those of the granules. The loss rates of chlorogenic acid, liquiritin, glycyrrhizin, and forsythoside A were higher than other ingredients in the whole process. In the AHP evaluation, yinqiaojiedu soft capsules got the highest score, followed by yinqiaojiedu tablets and yinqiaojiedu granules.CONCLUSION The concentrating-drying process is the key process of yinqiao analogus preparations. Among the three preparation process, the highest retention rate of chlorogenic acid is yinqiaojiedu tablets process. However, results of AHP evaluation for other active ingredients show that yinqiaojiedu soft capsules processing would be better than the other two.
关键词
银翘类方 /
绿原酸 /
甘草苷 /
甘草酸 /
提取-浓缩-干燥 /
损失率
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Key words
yinqiao analogus Preparations /
chlorogenic acid /
liquiritin /
glycyrrhizin /
extracting-concentrating-drying process chain /
loss rate
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中图分类号：
R284
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脚注
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基金
江西中医药大学校级科研课题(2012FC004);江西中医药大学校级研究生创新基金(JZYC14A07)
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